Osmotic stress response of the coral and oyster pathogen Vibrio coralliilyticus: acquisition of catabolism gene clusters for the compatible solute and signaling molecule myo-inositol | Semantic Scholar (2024)

It is demonstrated, by one-dimensional nuclear magnetic resonance analysis, that V. parahaemolyticus is capable of de novo synthesis of ectoine at high salinity whereas a ΔectB knockout strain is not.

A feed-forward loop wherein quorum sensing regulators AphA and OpaR integrate with the osmotic stress response pathway to control transcription of ectoine biosynthesis genes in V. parahaemolyticus is demonstrated and suggests that this mechanism may be widespread in Vibrio species.

This is the first report of MG and DIP in an actinobacterium and includes the identification of the new solute di-N-acetyl-glucosamine phosphate, which was always present and increased with the growth temperature.

It is shown that DMSP is used as an osmoprotectant by V. parahaemolyticus and by several other Vibrio species, including VIBrio cholerae and Vibria vulnificus, which suggests that DM SP is a significant bacterial osmOProtECTant that may be important for understanding the fate of DMSP in the environment.

13C-labelling highlighted differential antioxidant- and ammonium-producing pathway activities, suggesting physiological responses to different symbiont species, and contributes valuable information towards future scenarios that select in favour of less-competent symbionts in response to environmental change.

It is directly demonstrated that lipid droplets and glycogen granules in the coral tissue are sinks for translocated carbon photosynthates by dinoflagellates and confirm their key role in the trophic interactions within the coral-dinof lagellate association.

Autotrophic carbon fate within the free metabolite pools of both partners of a model cnidarian-dinoflagellate symbiosis is mapped during exposure to thermal stress via the stable isotope tracer (13 C bicarbonate) coupled to GC-MS.

Overall, it is demonstrated that DMG is a highly effective compatible solute for Vibrio species and the amino acid residues in BccT1 that are important for the coordination of GB, DMG, and ectoine transport are elucidated.

It is found that Corynebacterium glutamicum utilizes myo-inositol as a carbon and energy source, enabling proliferation with a high maximum rate of 0.35 h-1.35 mol/mol, and genome comparisons with other bacteria highlight the core genes required for growth on myo, inositol, whose metabolism is still weakly defined.

The results of this study indicate that V. coralliilyticus strain P1 has a diverse virulence repertoire that possibly enables this bacterium to be an efficient animal pathogen.

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